pgl3 u6 sgrna bfp Search Results


pgl3 u6 sgrna bfp  (Addgene inc)
88
Addgene inc pgl3 u6 sgrna bfp
ABE-mediated efficient A-to-G conversion at Ar and Hoxd13 loci in mouse embryos. a <t>GFP-to-BFP</t> conversion as a reporter for ABE-mediated base editing. b Analysis of base editing by FACS (left) and Sanger sequencing (right). Scramble: Scrambled <t>sgRNA</t> as negative control; sgRNA: sgRNA targeting GFP; PC: BFP expression plasmid only as positive control. Data were analyzed by Student’s t -test (*** p < 0.001) and shown as mean ± s.e.m. ( n = 3 from three independent experiments). c ABE-mediated base editing in vivo. ABE mRNA and sgRNA were co-injected into one-cell embryos, and the editing efficiency examined at blastocyst stage. d Efficiency of A-to-G substitution in mouse embryos. TA clones of PCR amplicons from the target regions in Ar (for sgAr-1, sgAr-15) and Hoxd13 (sgHoxd13) were analyzed by Sanger sequencing. Each dot indicates one individual mouse. At least 10 TA clones were analyzed for each sample. e The editing frequencies of individual A-to-G conversion of samples described in c were analyzed. A 3 , A 7 , C 5 , and T 11 indicate edited positions of the protospacer for sgAr-1; A 6 , A 8 , and A 9 indicate edited positions of the protospacer for sgHoxd13. f Representative alignments of modified sequences from embryos after microinjection of ABE mRNA and sgRNAs into one-cell embryos. The PAM sequences and substitutions are highlighted in red and blue, respectively; the target codons are underlined; N/N represents positive colonies out of the total sequenced
Pgl3 U6 Sgrna Bfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pgl3 u6 sgrna bfp/product/Addgene inc
Average 88 stars, based on 1 article reviews
pgl3 u6 sgrna bfp - by Bioz Stars, 2026-03
88/100 stars
  Buy from Supplier

Image Search Results


ABE-mediated efficient A-to-G conversion at Ar and Hoxd13 loci in mouse embryos. a GFP-to-BFP conversion as a reporter for ABE-mediated base editing. b Analysis of base editing by FACS (left) and Sanger sequencing (right). Scramble: Scrambled sgRNA as negative control; sgRNA: sgRNA targeting GFP; PC: BFP expression plasmid only as positive control. Data were analyzed by Student’s t -test (*** p < 0.001) and shown as mean ± s.e.m. ( n = 3 from three independent experiments). c ABE-mediated base editing in vivo. ABE mRNA and sgRNA were co-injected into one-cell embryos, and the editing efficiency examined at blastocyst stage. d Efficiency of A-to-G substitution in mouse embryos. TA clones of PCR amplicons from the target regions in Ar (for sgAr-1, sgAr-15) and Hoxd13 (sgHoxd13) were analyzed by Sanger sequencing. Each dot indicates one individual mouse. At least 10 TA clones were analyzed for each sample. e The editing frequencies of individual A-to-G conversion of samples described in c were analyzed. A 3 , A 7 , C 5 , and T 11 indicate edited positions of the protospacer for sgAr-1; A 6 , A 8 , and A 9 indicate edited positions of the protospacer for sgHoxd13. f Representative alignments of modified sequences from embryos after microinjection of ABE mRNA and sgRNAs into one-cell embryos. The PAM sequences and substitutions are highlighted in red and blue, respectively; the target codons are underlined; N/N represents positive colonies out of the total sequenced

Journal: Nature Communications

Article Title: Efficient generation of mouse models of human diseases via ABE- and BE-mediated base editing

doi: 10.1038/s41467-018-04768-7

Figure Lengend Snippet: ABE-mediated efficient A-to-G conversion at Ar and Hoxd13 loci in mouse embryos. a GFP-to-BFP conversion as a reporter for ABE-mediated base editing. b Analysis of base editing by FACS (left) and Sanger sequencing (right). Scramble: Scrambled sgRNA as negative control; sgRNA: sgRNA targeting GFP; PC: BFP expression plasmid only as positive control. Data were analyzed by Student’s t -test (*** p < 0.001) and shown as mean ± s.e.m. ( n = 3 from three independent experiments). c ABE-mediated base editing in vivo. ABE mRNA and sgRNA were co-injected into one-cell embryos, and the editing efficiency examined at blastocyst stage. d Efficiency of A-to-G substitution in mouse embryos. TA clones of PCR amplicons from the target regions in Ar (for sgAr-1, sgAr-15) and Hoxd13 (sgHoxd13) were analyzed by Sanger sequencing. Each dot indicates one individual mouse. At least 10 TA clones were analyzed for each sample. e The editing frequencies of individual A-to-G conversion of samples described in c were analyzed. A 3 , A 7 , C 5 , and T 11 indicate edited positions of the protospacer for sgAr-1; A 6 , A 8 , and A 9 indicate edited positions of the protospacer for sgHoxd13. f Representative alignments of modified sequences from embryos after microinjection of ABE mRNA and sgRNAs into one-cell embryos. The PAM sequences and substitutions are highlighted in red and blue, respectively; the target codons are underlined; N/N represents positive colonies out of the total sequenced

Article Snippet: Plasmids used include pGL3-U6-sgRNA-PGK-puromycin (Addgene, 51133), pUC57-sgRNA expression vector (Addgene, 51132), pCMV-SaBE3 (Addgene, 85169), pGFP-N1 (Addgene, 54712), pUC57-Sa sgRNA expression vector, pGL3-U6-sgRNA-EGFP, pGL3-U6-sgRNA-BFP, and pCMV-ABE.

Techniques: Sequencing, Negative Control, Expressing, Plasmid Preparation, Positive Control, In Vivo, Injection, Clone Assay, Modification